Sexual activity between women is as diverse as sex between heterosexuals or gay men. Some women in same-sex relationships do not identify as lesbian, but as bisexual, queer, or another label. As with any interpersonal activity, sexual expression depends on the context of the relationship.

Recent cultural changes in western and a few other societies have enabled lesbians to express their sexuality more freely, which has resulted in new studies on the nature of female sexuality. Research undertaken by the U.S. Government's National Center for Health Research in 2002 was released in a 2005 report called 'Sexual Behavior and Selected Health Measures: Men and Women 15-44 Years of Age, United States, 2002'. The results indicated that among women aged 15-44, 4.4 percent reported having had a sexual experience with another woman during the previous 12 months. When women aged 15–44 years of age were asked, "Have you ever had any sexual experience of any kind with another female?" 11 percent answered "yes".

There is a growing body of research and writing on lesbian sexuality, which has brought some debate about the control women have over their sexual lives, the fluidity of woman-to-woman sexuality, the redefinition of female sexual pleasure and the debunking of negative sexual stereotypes. One example of the latter is lesbian bed death, a term invented by sex researcher Pepper Schwartz to describe the supposedly inevitable diminution of sexual passion in long term lesbian relationships; this notion is rejected by many lesbians, who point out that passion tends to diminish in almost any relationship and many lesbian couples report happy and satisfying sex lives